Ultimate 3000 pumps

The Ultimate 3000 pumps family offers the most complete choice in the industry. From nano LC to rapid separation applications, from conventional applications to UHPLC, the UltiMate 3000 pumps always provide industry-leading flow, pressure, and precision.

Isocratic: Analytical

Binary: RSLCnano, Analytical, RSLC

Quaternary: Micro, Analytical, RSLC

Dual-Gradient: Micro, Analytical, RSLC


The Ultimate 3000 autosamplers ensure reliable, precise, and accurate injections of nL to mL sample volumes, with extremely low carry-overs. Fraction collection options range from simple collection to advanced collection-reinjection workflows such as automated offline 2D-LC.

Autosamplers: Split-Loop, Pulled-Loop with integrated column compartment

Fraction Collectors

Devices that perform UHPLC and HPLC purification on a variety of sample types and maintain sample integrity. Products are available in multiple sample capacity formats to accommodate various sample vessels and are compatible with common separation techniques

The System offers:

  • Biocompatible Version- Yes, pH range 2-12, chloride concentration up to 1 mol/L
  • Carryover-15 µL (standard), 1.37 µL (with optional 0.4 mm ID kit)
  • Flow Rate Range-0.4 mm ID drop former: 0.5 to 5 mL/min

                               1 mm ID drop former: 5 to 150 mL/min

Column Compartments

Therostatted column compartments and accessories designed for use with HPLC and UHPLC systems. Products allow for precise temperature control during liquid chromatography applications, thus minimizing performance loss from temperature effects.


Optical Detectors: Thermo Scientific optical detectors are available for UV-vis absorbance, fluorescence, and refractive-index detection of a wide variety of analytes.

Charged Aerosol Detectors:  Thermo Scientific charged aerosol detectors provide universal detection aerosol detectors provide universal detection of any non-volatile analyte and many semi volatile compounds, with or without chromaphores. Detectors are available for both the Vanquish UHPLC system and the Ultimate 3000 platform.

Electrochemical Detectors: Thermo Scientific Dionex Ultimate 3000 ECD-3000RS Electrochemical Detector offers easy, direct measurement to femtogram levels in vey limited sample volumes with minimal preparation. It readily eliminates matrix interferences to enhance selectivity. Users have a choice of both coulometric and amperometric sensors for extremely selective analyses using microbore to normal bore columns for both HPLC and UHPLC. This is the only electrochemical detector compatible with gradient chromatography.